Sequencing and cloning of antigen-specific antibodies from mouse memory B cells
Sequencing and cloning of antigen-specific antibodies from mouse memory B cells
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
In this protocol, the variable antibody region from antigen-specific mouse memory B cells is amplified and cloned into a constant region containing vectors by a sequence/ligation-independent method and used for the production of monoclonal antibodies.
Methods to identify genes encoding immunoglobulin heavy and light chains from single B lymphocy...
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Full title
Sequencing and cloning of antigen-specific antibodies from mouse memory B cells
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TN_cdi_proquest_miscellaneous_1859726509
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1859726509
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ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2016.102
