Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia...
Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia coli
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Publisher
New York: Nature Publishing Group US
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Language
English
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Publisher
New York: Nature Publishing Group US
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Scope and Contents
Contents
A method to screen proteins for enzymatic activity by incubating purified or overexpressed proteins with a metabolite extract and measuring changes in metabolite abundance using mass spectrometry enables high-throughput characterization of functionally uncharacterized proteins in
Escherichia coli
.
Our understanding of metabolism is limite...
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Full title
Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia coli
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TN_cdi_proquest_miscellaneous_1872818898
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1872818898
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ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth.4103