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Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia...

Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1872818898

Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia coli

About this item

Full title

Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia coli

Publisher

New York: Nature Publishing Group US

Journal title

Nature methods, 2017-02, Vol.14 (2), p.187-194

Language

English

Formats

Publication information

Publisher

New York: Nature Publishing Group US

More information

Scope and Contents

Contents

A method to screen proteins for enzymatic activity by incubating purified or overexpressed proteins with a metabolite extract and measuring changes in metabolite abundance using mass spectrometry enables high-throughput characterization of functionally uncharacterized proteins in
Escherichia coli
.
Our understanding of metabolism is limite...

Alternative Titles

Full title

Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia coli

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_miscellaneous_1872818898

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1872818898

Other Identifiers

ISSN

1548-7091

E-ISSN

1548-7105

DOI

10.1038/nmeth.4103

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