Cloning and Characterization of Cold-Adapted α-Amylase from Antarctic Arthrobacter agilis
Cloning and Characterization of Cold-Adapted α-Amylase from Antarctic Arthrobacter agilis
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Publisher
New York: Springer US
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Language
English
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New York: Springer US
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Contents
In this study, the gene encoding an α-amylase from a psychrophilic
Arthrobacter agilis
PAMC 27388 strain was cloned into a pET-28a(+) vector and heterologously expressed in
Escherichia coli
BL21(DE3). The recombinant α-amylase with a molecular mass of about 80 kDa was purified by using Ni
2+
-NTA affinity chromatography. This reco...
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Full title
Cloning and Characterization of Cold-Adapted α-Amylase from Antarctic Arthrobacter agilis
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TN_cdi_proquest_miscellaneous_1877840863
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1877840863
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ISSN
0273-2289
E-ISSN
1559-0291
DOI
10.1007/s12010-016-2267-5
