Fluorometric determination of RNase H via a DNAzyme conjugated to reduced graphene oxide, and its ap...
Fluorometric determination of RNase H via a DNAzyme conjugated to reduced graphene oxide, and its application to screening for inhibitors and activators
About this item
Full title
Author / Creator
Tong, Chunyi , Zhou, Ting , Zhao, Chuan , Yuan, Liqun , Xu, Ying , Liu, Bin , Fan, Jialong , Li, Dan and Zhu, Aiguo
Publisher
Vienna: Springer Vienna
Journal title
Language
English
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Publisher
Vienna: Springer Vienna
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Scope and Contents
Contents
A new fluorometric method is delineated for the detection of RNase H activity by combining DNAzyme with reduced graphene oxide (rGO). In the absence of RNase H, the fluorescence of FAM-labeled probe is quenched due to the strong adsorption on the rGO. The presence of RNase H can release the active DNAzyme from the DNA-RNA chimeric strand. This trig...
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Full title
Fluorometric determination of RNase H via a DNAzyme conjugated to reduced graphene oxide, and its application to screening for inhibitors and activators
Authors, Artists and Contributors
Author / Creator
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Primary Identifiers
Record Identifier
TN_cdi_proquest_miscellaneous_2231888774
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2231888774
Other Identifiers
ISSN
0026-3672
E-ISSN
1436-5073
DOI
10.1007/s00604-019-3425-6
