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CRISPR–Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes

CRISPR–Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2423064360

CRISPR–Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes

About this item

Full title

CRISPR–Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes

Publisher

London: Nature Publishing Group UK

Journal title

Nature protocols, 2020-08, Vol.15 (8), p.2470-2502

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

Streptomycetes are prominent sources of bioactive natural products, but metabolic engineering of the natural products of these organisms is greatly hindered by relatively inefficient genetic manipulation approaches. New advances in genome editing techniques, particularly CRISPR-based tools, have revolutionized genetic manipulation of many organisms...

Alternative Titles

Full title

CRISPR–Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_miscellaneous_2423064360

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2423064360

Other Identifiers

ISSN

1754-2189

E-ISSN

1750-2799

DOI

10.1038/s41596-020-0339-z

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