High-efficiency prime editing with optimized, paired pegRNAs in plants
High-efficiency prime editing with optimized, paired pegRNAs in plants
About this item
Full title
Author / Creator
Lin, Qiupeng , Jin, Shuai , Zong, Yuan , Yu, Hong , Zhu, Zixu , Liu, Guanwen , Kou, Liquan , Wang, Yanpeng , Qiu, Jin-Long , Li, Jiayang and Gao, Caixia
Publisher
New York: Nature Publishing Group US
Journal title
Language
English
Formats
Publication information
Publisher
New York: Nature Publishing Group US
Subjects
More information
Scope and Contents
Contents
Prime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30 °C leads to optimal performance in rice and that using two prime editing guide (peg) RNAs
in trans
encoding the same edits substantially enhances PE efficiency. Together, these approaches boos...
Alternative Titles
Full title
High-efficiency prime editing with optimized, paired pegRNAs in plants
Authors, Artists and Contributors
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Primary Identifiers
Record Identifier
TN_cdi_proquest_miscellaneous_2506284494
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2506284494
Other Identifiers
ISSN
1087-0156
E-ISSN
1546-1696
DOI
10.1038/s41587-021-00868-w
