An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells
An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells
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New York: Nature Publishing Group US
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English
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New York: Nature Publishing Group US
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Tandem affinity purification (TAP) is a generic two-step affinity purification protocol that enables the isolation of protein complexes under close-to-physiological conditions for subsequent analysis by mass spectrometry. Although TAP was instrumental in elucidating the yeast cellular machinery, in mammalian cells the method suffers from a low over...
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An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells
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TN_cdi_proquest_miscellaneous_68174241
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_68174241
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ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth968
