An optimized protocol for protein purification in cultured mammalian cells using a tandem affinity p...
An optimized protocol for protein purification in cultured mammalian cells using a tandem affinity purification approach
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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This protocol describes a method that we developed to adapt the tandem affinity purification (TAP) approach for use in mammalian cells. The protocol involves fusing a protein of interest with a tandem tag consisting of two FLAG tags (FF) followed by two protein-A immunoglobulin G (IgG) binding domains (ZZ). The protocol improves upon previously pub...
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An optimized protocol for protein purification in cultured mammalian cells using a tandem affinity purification approach
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TN_cdi_proquest_miscellaneous_68324273
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_68324273
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ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2006.371
