Log in to save to my catalogue

A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)

A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_68325612

A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)

About this item

Full title

A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)

Author / Creator

Publisher

London: Nature Publishing Group UK

Journal title

Nature protocols, 2007-01, Vol.1 (6), p.2650-2660

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

Stable isotope labeling by amino acids in cell culture (SILAC) is a simple, robust, yet powerful approach in mass spectrometry (MS)-based quantitative proteomics. SILAC labels cellular proteomes through normal metabolic processes, incorporating non-radioactive, stable isotope-containing amino acids in newly synthesized proteins. Growth medium is pr...

Alternative Titles

Full title

A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_miscellaneous_68325612

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_68325612

Other Identifiers

ISSN

1754-2189

E-ISSN

1750-2799

DOI

10.1038/nprot.2006.427

How to access this item