Synthesis of biotin–AMP conjugate for 5′ biotin labeling of RNA through one-step in vitro transcript...
Synthesis of biotin–AMP conjugate for 5′ biotin labeling of RNA through one-step in vitro transcription
About this item
Full title
Author / Creator
Huang, Faqing , He, Jun , Zhang, Yilin and Guo, Yanlin
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
Formats
Publication information
Publisher
London: Nature Publishing Group UK
Subjects
More information
Scope and Contents
Contents
Biotin-labeled RNA has found broad applications in chemistry, biology and biomedicine. In this protocol, we describe a simple procedure for 5′ RNA biotin labeling by one-step
in vitro
transcription. A biotin–AMP (adenosine 5′-monophosphate) conjugate, biotin-HDAAMP (adenosine 5′-(6-aminohexyl) phosphoramide; where HDA is 1,6-hexanediamine), i...
Alternative Titles
Full title
Synthesis of biotin–AMP conjugate for 5′ biotin labeling of RNA through one-step in vitro transcription
Authors, Artists and Contributors
Author / Creator
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_proquest_miscellaneous_69769133
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_69769133
Other Identifiers
ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2008.185
