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improved one-tube RT-PCR protocol for analyzing single-cell gene expression in individual mammalian...

improved one-tube RT-PCR protocol for analyzing single-cell gene expression in individual mammalian...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_753652938

improved one-tube RT-PCR protocol for analyzing single-cell gene expression in individual mammalian cells

About this item

Full title

improved one-tube RT-PCR protocol for analyzing single-cell gene expression in individual mammalian cells

Publisher

Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag

Journal title

Analytical and bioanalytical chemistry, 2010-07, Vol.397 (5), p.1853-1859

Language

English

Formats

Publication information

Publisher

Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag

More information

Scope and Contents

Contents

It is well known that gene expression is regulated at the level of individual cells, and more evidence is now emerging that heterogeneity of cell regulation is orders of magnitude greater than previously thought. In order to detect meaningful variations in transcription levels, it is necessary to measure gene expression at single cell levels rather...

Alternative Titles

Full title

improved one-tube RT-PCR protocol for analyzing single-cell gene expression in individual mammalian cells

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_proquest_miscellaneous_753652938

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_753652938

Other Identifiers

ISSN

1618-2642

E-ISSN

1618-2650

DOI

10.1007/s00216-010-3754-0

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