Breaking the Diffraction Barrier in Fluorescence Microscopy at Low Light Intensities by Using Revers...
Breaking the Diffraction Barrier in Fluorescence Microscopy at Low Light Intensities by Using Reversibly Photoswitchable Proteins
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United States: National Academy of Sciences
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English
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United States: National Academy of Sciences
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Fluorescence microscopy is indispensable in many areas of science, but until recently, diffraction has limited the resolution of its lens-based variant. The diffraction barrier has been broken by a saturated depletion of the marker's fluorescent state by stimulated emission, but this approach requires picosecond laser pulses of$GW/cm^2$intensity. H...
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Breaking the Diffraction Barrier in Fluorescence Microscopy at Low Light Intensities by Using Reversibly Photoswitchable Proteins
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TN_cdi_pubmed_primary_16314572
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmed_primary_16314572
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0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.0506010102