1 H-NMR spectroscopy shows cellular uptake of HEPES buffer by human cell lines-an effect to be consi...
1 H-NMR spectroscopy shows cellular uptake of HEPES buffer by human cell lines-an effect to be considered in cell culture experiments
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Publisher
Germany
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Language
English
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Publisher
Germany
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Contents
HEPES is commonly used in cell culture media as a buffering substance. Compared to the bicarbonate/CO
buffer system, it does not require a CO
atmosphere, thereby ensuring stable pH values during handling of cell culture media outside of an incubator. Due to its intrinsic charge, HEPES is considered not to be taken up by cells, which was a pre...
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Full title
1 H-NMR spectroscopy shows cellular uptake of HEPES buffer by human cell lines-an effect to be considered in cell culture experiments
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TN_cdi_pubmed_primary_30506504
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmed_primary_30506504
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E-ISSN
1618-2650
DOI
10.1007/s00216-018-1518-4
