CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering
CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering
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Publisher
New York: Nature Publishing Group US
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Language
English
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Publisher
New York: Nature Publishing Group US
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Contents
Existing technologies for site-specific integration of kilobase-sized DNA sequences in bacteria are limited by low efficiency, a reliance on recombination, the need for multiple vectors, and challenges in multiplexing. To address these shortcomings, we introduce a substantially improved version of our previously reported Tn
7
-like transposon...
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Full title
CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10583764
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10583764
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ISSN
1087-0156
E-ISSN
1546-1696
DOI
10.1038/s41587-020-00745-y