Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and hu...
Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain
About this item
Full title
Author / Creator
Marsh, Samuel E. , Walker, Alec J. , Kamath, Tushar , Dissing-Olesen, Lasse , Hammond, Timothy R. , de Soysa, T. Yvanka , Young, Adam M. H. , Murphy, Sarah , Abdulraouf, Abdulraouf , Nadaf, Naeem , Dufort, Connor , Walker, Alicia C. , Lucca, Liliana E. , Kozareva, Velina , Vanderburg, Charles , Hong, Soyon , Bulstrode, Harry , Hutchinson, Peter J. , Gaffney, Daniel J. , Hafler, David A. , Franklin, Robin J. M. , Macosko, Evan Z. and Stevens, Beth
Publisher
New York: Nature Publishing Group US
Journal title
Language
English
Formats
Publication information
Publisher
New York: Nature Publishing Group US
Subjects
More information
Scope and Contents
Contents
A key aspect of nearly all single-cell sequencing experiments is dissociation of intact tissues into single-cell suspensions. While many protocols have been optimized for optimal cell yield, they have often overlooked the effects that dissociation can have on ex vivo gene expression. Here, we demonstrate that use of enzymatic dissociation on brain...
Alternative Titles
Full title
Dissection of artifactual and confounding glial signatures by single-cell sequencing of mouse and human brain
Authors, Artists and Contributors
Author / Creator
Walker, Alec J.
Kamath, Tushar
Dissing-Olesen, Lasse
Hammond, Timothy R.
de Soysa, T. Yvanka
Young, Adam M. H.
Murphy, Sarah
Abdulraouf, Abdulraouf
Nadaf, Naeem
Dufort, Connor
Walker, Alicia C.
Lucca, Liliana E.
Kozareva, Velina
Vanderburg, Charles
Hong, Soyon
Bulstrode, Harry
Hutchinson, Peter J.
Gaffney, Daniel J.
Hafler, David A.
Franklin, Robin J. M.
Macosko, Evan Z.
Stevens, Beth
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11645269
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11645269
Other Identifiers
ISSN
1097-6256,1546-1726
E-ISSN
1546-1726
DOI
10.1038/s41593-022-01022-8
