A Fluorescence-Based Technique to Construct Size Distributions from Single-Object Measurements: Appl...
A Fluorescence-Based Technique to Construct Size Distributions from Single-Object Measurements: Application to the Extrusion of Lipid Vesicles
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Publisher
United States: Elsevier Inc
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Language
English
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Publisher
United States: Elsevier Inc
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Contents
We report a novel approach to quantitatively determine complete size distributions of surface-bound objects using fluorescence microscopy. We measure the integrated intensity of single particles and relate it to their size by taking into account the object geometry and the illumination profile of the microscope, here a confocal laser scanning micro...
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Full title
A Fluorescence-Based Technique to Construct Size Distributions from Single-Object Measurements: Application to the Extrusion of Lipid Vesicles
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2479610
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2479610
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ISSN
0006-3495
E-ISSN
1542-0086
DOI
10.1529/biophysj.108.128819