Kinetic flux profiling for quantitation of cellular metabolic fluxes
Kinetic flux profiling for quantitation of cellular metabolic fluxes
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Scope and Contents
Contents
This protocol enables quantitation of metabolic fluxes in cultured cells. Measurements are based on the kinetics of cellular incorporation of stable isotope from nutrient into downstream metabolites. At multiple time points, after cells are rapidly switched from unlabeled to isotope-labeled nutrient, metabolism is quenched, metabolites are extracte...
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Full title
Kinetic flux profiling for quantitation of cellular metabolic fluxes
Authors, Artists and Contributors
Author / Creator
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Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2710581
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2710581
Other Identifiers
ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2008.131
