The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry
The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Scope and Contents
Contents
The success in profiling the phosphoproteome by mass spectrometry-based proteomics has been intimately related to the availability of methods that selectively enrich for phosphopeptides. To this end, we describe a protocol that combines two sequential enrichment steps. First, strong cation exchange (SCX) chromatography separates peptides by solutio...
Alternative Titles
Full title
The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry
Authors, Artists and Contributors
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2728452
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2728452
Other Identifiers
ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2008.150
