Tryptophan-based chromophore in fluorescent proteins can be anionic
Tryptophan-based chromophore in fluorescent proteins can be anionic
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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Cyan fluorescent proteins (CFP) with tryptophan66-based chromophore are widely used for live cell imaging. In contrast to green and red fluorescent proteins, no charged states of the CFP chromophore have been described. Here, we studied synthetic CFP chromophore and found that its indole group can be deprotonated rather easily (pKa 12.4).We then re...
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Full title
Tryptophan-based chromophore in fluorescent proteins can be anionic
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3429880
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3429880
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ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/srep00608
