SDM-Assist software to design site-directed mutagenesis primers introducing "silent" restriction sit...
SDM-Assist software to design site-directed mutagenesis primers introducing "silent" restriction sites
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Publisher
England: BioMed Central Ltd
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Language
English
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England: BioMed Central Ltd
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Scope and Contents
Contents
Over the past decades site-directed mutagenesis (SDM) has become an indispensable tool for biological structure-function studies. In principle, SDM uses modified primer pairs in a PCR reaction to introduce a mutation in a cDNA insert. DpnI digestion of the reaction mixture is used to eliminate template copies before amplification in E. coli; howeve...
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Full title
SDM-Assist software to design site-directed mutagenesis primers introducing "silent" restriction sites
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3644487
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3644487
Other Identifiers
ISSN
1471-2105
E-ISSN
1471-2105
DOI
10.1186/1471-2105-14-105
