Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag‐induce...
Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag‐induced reporter fluorescence
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Publisher
Oxford, UK: Blackwell Publishing Ltd
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Language
English
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Publisher
Oxford, UK: Blackwell Publishing Ltd
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Summary
An intracellular approach for monitoring protein production in Staphylococcus aureus is described. mCherry, fused to the dodecapeptide Tip, was capable of inducing tetracycline repressor (TetR). Time‐ and concentration‐dependent production of mCherry could be correlated to TetR‐controlled GFPmut2 activity. This approach can potentially b...
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Full title
Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag‐induced reporter fluorescence
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Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3815279
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3815279
Other Identifiers
ISSN
1751-7915
E-ISSN
1751-7915
DOI
10.1111/j.1751-7915.2011.00304.x