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Avoiding the ends: internal epitope tagging of proteins using transposon Tn7

Avoiding the ends: internal epitope tagging of proteins using transposon Tn7

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4423380

Avoiding the ends: internal epitope tagging of proteins using transposon Tn7

About this item

Full title

Avoiding the ends: internal epitope tagging of proteins using transposon Tn7

Publisher

United States: Genetics Society of America

Journal title

Genetics (Austin), 2015-05, Vol.200 (1), p.47-58

Language

English

Formats

Publication information

Publisher

United States: Genetics Society of America

More information

Scope and Contents

Contents

Peptide tags fused to proteins are used in a variety of applications, including as affinity tags for purification, epitope tags for immunodetection, or fluorescent protein tags for visualization. However, the peptide tags can disrupt the target protein function. When function is disrupted by fusing a peptide to either the N or C terminus of the pro...

Alternative Titles

Full title

Avoiding the ends: internal epitope tagging of proteins using transposon Tn7

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4423380

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4423380

Other Identifiers

ISSN

1943-2631,0016-6731

E-ISSN

1943-2631

DOI

10.1534/genetics.114.169482

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