Avoiding the ends: internal epitope tagging of proteins using transposon Tn7
Avoiding the ends: internal epitope tagging of proteins using transposon Tn7
About this item
Full title
Author / Creator
Publisher
United States: Genetics Society of America
Journal title
Language
English
Formats
Publication information
Publisher
United States: Genetics Society of America
Subjects
More information
Scope and Contents
Contents
Peptide tags fused to proteins are used in a variety of applications, including as affinity tags for purification, epitope tags for immunodetection, or fluorescent protein tags for visualization. However, the peptide tags can disrupt the target protein function. When function is disrupted by fusing a peptide to either the N or C terminus of the pro...
Alternative Titles
Full title
Avoiding the ends: internal epitope tagging of proteins using transposon Tn7
Authors, Artists and Contributors
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4423380
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4423380
Other Identifiers
ISSN
1943-2631,0016-6731
E-ISSN
1943-2631
DOI
10.1534/genetics.114.169482