Identifying and mitigating bias in next-generation sequencing methods for chromatin biology
Identifying and mitigating bias in next-generation sequencing methods for chromatin biology
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
Key Points
In next-generation sequencing (NGS) chromatin profiling experiments technical artefacts may be introduced at any stage, most importantly in fragmenting DNA, selecting the fragment population of interest, DNA amplification, DNA sequencing itself and read mapping to a reference genome.
The effect of technical biases on experimental r...
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Full title
Identifying and mitigating bias in next-generation sequencing methods for chromatin biology
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4473780
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4473780
Other Identifiers
ISSN
1471-0056
E-ISSN
1471-0064
DOI
10.1038/nrg3788
