Application of BRET to monitor ligand binding to GPCRs
Application of BRET to monitor ligand binding to GPCRs
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Publisher
New York: Nature Publishing Group US
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Language
English
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Publisher
New York: Nature Publishing Group US
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Contents
Bioluminescence resonance energy transfer, from the substrate of a luciferase fused with a G protein–coupled receptor to a fluorescent dye covalently linked to a receptor ligand, allows the profiling of ligand affinity and binding kinetics.
Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-pr...
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Full title
Application of BRET to monitor ligand binding to GPCRs
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4488387
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4488387
Other Identifiers
ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth.3398
