Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics
Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
Unculturable bacterial communities provide a rich source of biocatalysts, but their experimental discovery by functional metagenomics is difficult, because the odds are stacked against the experimentor. Here we demonstrate functional screening of a million-membered metagenomic library in microfluidic picolitre droplet compartments. Using bait subst...
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Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4686663
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4686663
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/ncomms10008
