Phased diploid genome assembly with single-molecule real-time sequencing
Phased diploid genome assembly with single-molecule real-time sequencing
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Author / Creator
Chin, Chen-Shan , Peluso, Paul , Sedlazeck, Fritz J , Nattestad, Maria , Concepcion, Gregory T , Clum, Alicia , Dunn, Christopher , O'Malley, Ronan , Figueroa-Balderas, Rosa , Morales-Cruz, Abraham , Cramer, Grant R , Delledonne, Massimo , Luo, Chongyuan , Ecker, Joseph R , Cantu, Dario , Rank, David R and Schatz, Michael C
Publisher
New York: Nature Publishing Group US
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English
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New York: Nature Publishing Group US
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Contents
The open-source FALCON and FALCON-Unzip software utilize long-read sequencing data to generate contiguous, accurate and phased diploid assemblies, even from genomes that are highly heterozygous.
While genome assembly projects have been successful in many haploid and inbred species, the assembly of noninbred or rearranged heterozygous genomes remains a major challenge. To address this challenge, we introduce the open-source FALCON and FALCON-Unzip algorithms (
https://github.com/PacificBiosciences/FALCON/
) to assemble long-read sequencing data into highly accurate, contiguous, and correctly phased diploid genomes. We generate new reference sequences for heterozygous samples including an F1 hybrid of
Arabidopsis thaliana
, the widely cultivated
Vitis vinifera
cv. Cabernet Sauvignon, and the coral fungus
Clavicorona pyxidata
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Full title
Phased diploid genome assembly with single-molecule real-time sequencing
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5503144
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5503144
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ISSN
1548-7091,1548-7105
E-ISSN
1548-7105
DOI
10.1038/nmeth.4035
