Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells
Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells
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Publisher
New York: Nature Publishing Group US
Journal title
Language
English
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Publisher
New York: Nature Publishing Group US
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Scope and Contents
Contents
Recruiting a hyperactive cytidine deaminase via the guide RNA to dCas9 allows for the introduction of diverse point mutations at the CRISPR target locus to create complex libraries of variants for protein engineering or dissection of protein function.
Engineering and study of protein function by directed evolution has been limited by the technic...
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Full title
Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5557288
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5557288
Other Identifiers
ISSN
1548-7091,1548-7105
E-ISSN
1548-7105
DOI
10.1038/nmeth.4038
