PECAN: library-free peptide detection for data-independent acquisition tandem mass spectrometry data
PECAN: library-free peptide detection for data-independent acquisition tandem mass spectrometry data
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New York: Nature Publishing Group US
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English
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New York: Nature Publishing Group US
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A library-free, peptide-centric search tool, PECAN, robustly identifies peptides from data-independent acquisition mass-spectrometry-based proteomics data.
Data-independent acquisition (DIA) is an emerging mass spectrometry (MS)-based technique for unbiased and reproducible measurement of protein mixtures. DIA tandem mass spectrometry spectra are often highly multiplexed, containing product ions from multiple cofragmenting precursors. Detecting peptides directly from DIA data is therefore challenging; most DIA data analyses require spectral libraries. Here we present PECAN (
http://pecan.maccosslab.org
), a library-free, peptide-centric tool that robustly and accurately detects peptides directly from DIA data. PECAN reports evidence of detection based on product ion scoring, which enables detection of low-abundance analytes with poor precursor ion signal. We demonstrate the chromatographic peak picking accuracy and peptide detection capab...
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PECAN: library-free peptide detection for data-independent acquisition tandem mass spectrometry data
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5578911
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5578911
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ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth.4390
