Prokaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using...
Prokaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using maltose-binding protein
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
Human fibroblast growth factor 21 (hFGF21) has been characterized as an important regulator of glucose and lipid metabolism homeostasis. Here, to produce hFGF21 efficiently in
Escherichia coli
, the expression and solubility of hFGF21 were tested and optimised by fusing the protein with one of eight tags: hexahistidine (His6), thioredoxin (Tr...
Alternative Titles
Full title
Prokaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using maltose-binding protein
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5700921
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5700921
Other Identifiers
ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/s41598-017-16167-x
