CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets
CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets
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Publisher
New York: Nature Publishing Group US
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Language
English
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Publisher
New York: Nature Publishing Group US
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Contents
CIRCLE-seq is an
in vitro
assay for selectively sequencing off-target sites cleaved by Cas9–sgRNA in genomic DNA. It sensitively profiles genome-wide off-target cut sites and characterizes the contribution of SNPs to these cleavage events.
Sensitive detection of off-target effects is important for translating CRISPR–Cas9 nucleases into hum...
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Full title
CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5924695
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5924695
Other Identifiers
ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth.4278
