Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
About this item
Full title
Author / Creator
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
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Publication information
Publisher
London: Nature Publishing Group UK
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Scope and Contents
Contents
A key limitation of the use of the CRISPR–Cas9 system for genome editing and other applications is the requirement that a protospacer adjacent motif (PAM) be present at the target site. For the most commonly used Cas9 from
Streptococcus pyogenes
(
Sp
Cas9), the required PAM sequence is NGG. No natural or engineered Cas9 variants that ha...
Alternative Titles
Full title
Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
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Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5951633
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5951633
Other Identifiers
ISSN
0028-0836
E-ISSN
1476-4687
DOI
10.1038/nature26155
