Conditional reprogramming and long-term expansion of normal and tumor cells from human biospecimens
Conditional reprogramming and long-term expansion of normal and tumor cells from human biospecimens
About this item
Full title
Author / Creator
Liu, Xuefeng , Krawczyk, Ewa , Suprynowicz, Frank A , Palechor-Ceron, Nancy , Yuan, Hang , Dakic, Aleksandra , Simic, Vera , Zheng, Yun-Ling , Sripadhan, Praathibha , Chen, Chen , Lu, Jie , Hou, Tung-Wei , Choudhury, Sujata , Kallakury, Bhaskar , Tang, Dean G , Darling, Thomas , Thangapazham, Rajesh , Timofeeva, Olga , Dritschilo, Anatoly , Randell, Scott H , Albanese, Christopher , Agarwal, Seema and Schlegel, Richard
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
Formats
Publication information
Publisher
London: Nature Publishing Group UK
Subjects
More information
Scope and Contents
Contents
This protocol demonstrates how to establish primary epithelial cell cultures
in vitro
from healthy human tissue and human cancer samples using ROCK inhibitor and irradiated feeder cells.
Historically, it has been difficult to propagate cells
in vitro
that are derived directly from human tumors or healthy tissue. However,
in vitro<...
Alternative Titles
Full title
Conditional reprogramming and long-term expansion of normal and tumor cells from human biospecimens
Authors, Artists and Contributors
Author / Creator
Krawczyk, Ewa
Suprynowicz, Frank A
Palechor-Ceron, Nancy
Yuan, Hang
Dakic, Aleksandra
Simic, Vera
Zheng, Yun-Ling
Sripadhan, Praathibha
Chen, Chen
Lu, Jie
Hou, Tung-Wei
Choudhury, Sujata
Kallakury, Bhaskar
Tang, Dean G
Darling, Thomas
Thangapazham, Rajesh
Timofeeva, Olga
Dritschilo, Anatoly
Randell, Scott H
Albanese, Christopher
Agarwal, Seema
Schlegel, Richard
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6195120
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6195120
Other Identifiers
ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2016.174
