Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
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Publisher
England: John Wiley & Sons, Inc
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Language
English
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Publisher
England: John Wiley & Sons, Inc
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Summary
CRISPR/Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing tools in plants. In this work, we compared their activities by targeting maize glossy2 gene coding region that has overlapping sequences recognized by both nucleases. We introduced constructs carrying SpCas9‐guide RNA (gRNA) and LbCas12a‐CRISPR RNA (crRNA...
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Full title
Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6320322
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6320322
Other Identifiers
ISSN
1467-7644
E-ISSN
1467-7652
DOI
10.1111/pbi.12982
