Propidium iodide staining underestimates viability of adherent bacterial cells
Propidium iodide staining underestimates viability of adherent bacterial cells
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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Combining membrane impermeable DNA-binding stain propidium iodide (PI) with membrane-permeable DNA-binding counterstains is a widely used approach for bacterial viability staining. In this paper we show that PI staining of adherent cells in biofilms may significantly underestimate bacterial viability due to the presence of extracellular nucleic aci...
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Full title
Propidium iodide staining underestimates viability of adherent bacterial cells
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6482146
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6482146
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ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/s41598-019-42906-3
