Transcription-coupled changes in nuclear mobility of mammalian cis-regulatory elements
Transcription-coupled changes in nuclear mobility of mammalian cis-regulatory elements
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Publisher
United States: The American Association for the Advancement of Science
Journal title
Language
English
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United States: The American Association for the Advancement of Science
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Contents
To achieve guide RNA (gRNA) multiplexing and an efficient delivery of tens of distinct gRNAs into single cells, we developed a molecular assembly strategy termed chimeric array of gRNA oligonucleotides (CARGO). We coupled CARGO with dCas9 (catalytically dead Cas9) imaging to quantitatively measure the movement of enhancers and promoters that underg...
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Full title
Transcription-coupled changes in nuclear mobility of mammalian cis-regulatory elements
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6590518
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6590518
Other Identifiers
ISSN
0036-8075
E-ISSN
1095-9203
DOI
10.1126/science.aao3136
