Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor
Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor
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Publisher
New York: Nature Publishing Group US
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Language
English
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New York: Nature Publishing Group US
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Here we describe TRACE (T7 polymerase-driven continuous editing), a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations in genes under the control of a T7 promoter integrated in the genome. We used TRACE in a...
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Full title
Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7775643
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7775643
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ISSN
1087-0156
E-ISSN
1546-1696
DOI
10.1038/s41587-019-0331-8
