Laboratory evolution of a sortase enzyme that modifies amyloid-β protein
Laboratory evolution of a sortase enzyme that modifies amyloid-β protein
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Publisher
New York: Nature Publishing Group US
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Language
English
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Publisher
New York: Nature Publishing Group US
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Contents
Epitope-specific enzymes are powerful tools for site-specific protein modification but generally require genetic manipulation of the target protein. Here, we describe the laboratory evolution of the bacterial transpeptidase sortase A to recognize the LMVGG sequence in endogenous amyloid-β (Aβ) protein. Using a yeast display selection for covalent b...
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Full title
Laboratory evolution of a sortase enzyme that modifies amyloid-β protein
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7904614
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7904614
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ISSN
1552-4450
E-ISSN
1552-4469
DOI
10.1038/s41589-020-00706-1
