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Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8053421

Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

About this item

Full title

Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

Publisher

London: Nature Publishing Group UK

Journal title

Nature protocols, 2020-06, Vol.15 (6), p.2041-2070

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

Recent advances have made cryogenic (cryo) electron microscopy a key technique to achieve near-atomic-resolution structures of biochemically isolated macromolecular complexes. Cryo-electron tomography (cryo-ET) can give unprecedented insight into these complexes in the context of their natural environment. However, the application of cryo-ET is lim...

Alternative Titles

Full title

Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8053421

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8053421

Other Identifiers

ISSN

1754-2189

E-ISSN

1750-2799

DOI

10.1038/s41596-020-0320-x

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