Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue
Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue
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Author / Creator
Hardwick, Simon A. , Hu, Wen , Joglekar, Anoushka , Fan, Li , Collier, Paul G. , Foord, Careen , Balacco, Jennifer , Lanjewar, Samantha , Sampson, Maureen McGuirk , Koopmans, Frank , Prjibelski, Andrey D. , Mikheenko, Alla , Belchikov, Natan , Jarroux, Julien , Lucas, Anne Bergstrom , Palkovits, Miklós , Luo, Wenjie , Milner, Teresa A. , Ndhlovu, Lishomwa C. , Smit, August B. , Trojanowski, John Q. , Lee, Virginia M. Y. , Fedrigo, Olivier , Sloan, Steven A. , Tombácz, Dóra , Ross, M. Elizabeth , Jarvis, Erich , Boldogkői, Zsolt , Gan, Li and Tilgner, Hagen U.
Publisher
New York: Nature Publishing Group US
Journal title
Language
English
Formats
Publication information
Publisher
New York: Nature Publishing Group US
Subjects
More information
Scope and Contents
Contents
Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics, PCR-based artifact removal, target enrichment and...
Alternative Titles
Full title
Single-nuclei isoform RNA sequencing unlocks barcoded exon connectivity in frozen brain tissue
Authors, Artists and Contributors
Author / Creator
Hu, Wen
Joglekar, Anoushka
Fan, Li
Collier, Paul G.
Foord, Careen
Balacco, Jennifer
Lanjewar, Samantha
Sampson, Maureen McGuirk
Koopmans, Frank
Prjibelski, Andrey D.
Mikheenko, Alla
Belchikov, Natan
Jarroux, Julien
Lucas, Anne Bergstrom
Palkovits, Miklós
Luo, Wenjie
Milner, Teresa A.
Ndhlovu, Lishomwa C.
Smit, August B.
Trojanowski, John Q.
Lee, Virginia M. Y.
Fedrigo, Olivier
Sloan, Steven A.
Tombácz, Dóra
Ross, M. Elizabeth
Jarvis, Erich
Boldogkői, Zsolt
Gan, Li
Tilgner, Hagen U.
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9287170
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9287170
Other Identifiers
ISSN
1087-0156
E-ISSN
1546-1696
DOI
10.1038/s41587-022-01231-3
