猴头菌锰过氧化物酶1基因在构巢曲霉的异源转化与表达
猴头菌锰过氧化物酶1基因在构巢曲霉的异源转化与表达
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东北林业大学林学院,黑龙江哈尔滨150040
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为提高猴头菌菌株CB1锰过氧化物酶（MnP）基因的表达产量,采用PEG/CaCl2介导的原生质体转化方法,将携带有He-mnp1的重组质粒pLB01/He-mnp1转入到构巢曲霉尿嘧啶尿苷营养缺陷菌株TN02A7的原生质体中,获得了转化子菌株TN02A7-He-mnp1,并在乙醇脱氢酶启动子alcA（p）控制下实现了异源表达。将TN02A7-He-mnp1、TN02A7、构巢曲霉野生型菌株WJA01、猴头菌菌株CB1在相同的木质素环境下进行培养并检测MnP酶活性,结果表明：转化子菌株TN02A7-He-mnp1在0.05 g.L-1血红素的情况下、诱导96 h后酶活性最高为38.31 U.L-1,比不添加血红素的酶活力高8.64倍,但比猴头菌菌株CB1的酶活力低,而TN02A7与WJA01始...
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猴头菌锰过氧化物酶1基因在构巢曲霉的异源转化与表达
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TN_cdi_wanfang_journals_lykxyj201304014
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_wanfang_journals_lykxyj201304014
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1001-1498
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