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Numbers in brackets = original Walkabout negative numbers
OPAL MINING
1-2. Opal fields, Lightning Ridge / Australian National Travel Assoc. (4272 4273)
3-5. Opal fields, Lightning Ridge, July 1963 / Allan A.  Hedges Pty. Limited for NSW Govt. Tourist Bureau (8679 8683 8684)
6. Opal fields, Lightning Ridge / Australian Tourist Commission (1442-9)
BUILDINGS & MONUMENTS
7. Wingecarribee, Oxley Hill, Bowral, 1948 / R.Bean photographer (G2587)
8. [Hampden] Bridge, Kangaroo Valley /  (P.6850)
9. War Memorial Cultural Centre [Newcastle], 1959 / (2787)
10-11. [Courthouse and Post Office, Bathurst, 1949, [published Walkabout Aug 1965] / R.Bean (G 2861)
12. Dome of Courthouse in Bathurst, 1959 / Kevin L. Aston (B 221)
13-14. Courthouse and Post Office, 1963 / Allan Hedges (1723 2235)
15-16. Macquarie's house, 1 George St, Bathurst, oldest building west of the Blue Mountains, Oct-Nov 1966 / ANTA (film no. 442-exp. 4-1966)
17-18. Evans Memorial and War Memorial Carillon, Bathurst, July 1963 / Allan Hedges per NSWGTB (8776,8778)
19. Ben Boyd whaling tower, Eden, Oct 1947 / P.Payens (G 2578)
20. Ruins of church, Boydtown, Twofold Bay, n.d. / (P 4347)
21. Hotel, Boydtown, Oct 1965 / David Beal for ANTA (film no. 1-exp. 3-1965)
22. St. Matthews Church, Windsor / (R 1100)
23. Braidwood Roman Catholic Church [and grave memorial of Michael D'Arcy (d. 1905 aged 64 ?)] / David Beal for ANTA (ANTA no. 280/12)
24. [Church and campus], New England University, Armidale, Dec 1963 / NSWGTB (2220)
25. Church near Kangaloon, Southern Highlands / NSWGTB (300)
26. Camden Valley Inn near Camden, 9 May 1966 / (film no. 276-exp. 32-1966)
27. Eric Worrell's Reptile Park, Gosford, July 1963 / NSWGTB (6567)
28. Dog on the Tucker Box Pioneer Monument, Gundagai, July 1965 / Douglass Baglin Pty. Ltd. (3223)
29. Mail-Coach Office, Sandwych St, Wentworth, 1947 / (3200)
30. Surveyor General Hotel, Berrima first licensed 1835, the oldest NSW country license, 9 May 1966 / (film no. 275-exp. 17A-1966)
JEFF CARTER GAME FISHING
31. Veteran game fisherman William ("Billy") Love
32. Veteran game fisherman and rod-maker William Southam
33. Lord Mayor Jensen opens All Australia Game Fishing Contest for Australia Day, Watsons Bay wharf, n.d.
34-38. Claude Kellion and son John on Jac III with crewman John McIntyre / (no. 34 is 1327)
39-47. Bob and Dolly Dyer fish from their boat Tennessee II, 1963-196 / (1328 1946 2637 2638 2639 and unnumbered)
48-51. George and Patti Hoag of Newport, California, marlin fishing, Port Stephens, Aug 1964 / J.Carter (2641 2642 2643 2645)
52. Weighing a shark at the Sydney Game Fishing Club weighing station, Mar 1963 / (1329)
53. Landing a marlin onto Kathy II (boat), n.d.
54. Chevron II and game fishing boats cross the Heads, Nar 1963 / (1326)
55. Fishing boat Signa, Port Stephens, Aug 1964 / (2640)
56. Coxinga below the cliffs at Diamond Bay, n.d. / Jeff Carter
57. Shark caught by R.Compton in "Sea Lark" on the scales, Port Stephens, Aug 1964 / (2644)
58-61. Game fishing, Aug 1964 / (2632 2633 2635 2636)
62-69. Miscellaneous game fishing
AUSTRALIAN BUSH
70. Snowgums and Alpine flowers, 1967 / Helmut Gritscher
71. "From these slopes on Mount Kaputar, a wonderful view can be had, of the Nandwahs [i.e. Nandewars] looking to the east, and to the west, the north-west plains" / Barbara Hamilton
GOLF AND OUTDOOR FUNCTION
72-72a. Golfing at Nundle, N.S.W. / (8727)
73. [Unidentified outdoor function in bush clearing with marquee, table under umbrellas and bush band / Photograophic Illustrators (3381 3386)

Item 14: Walkabout magazine : New South Wales photographs [opal mining; early NSW buildings; fishing; bush and golf]

Activating mutations in the
B-Raf proto-oncogene serine/threonine kinase
(
BRAF
) gene occur in many tumor types, the highest incidence being in malignant melanoma and papillary thyroid carcinoma. In patients with
BRAF
mutations tumor progression is more rapid than in patients without these mutations. Therapeutic strategies presently aim at inhibiting BRAF resulting in slower tumor progression; however, lasting remission is rarely accomplished. In this paper we identify the oncomiR-3151 as a downstream effector of mutated
BRAF.
MicroRNA-3151 (miR-3151) targets TP53 and other members of the
TP53
pathway resulting in its inhibition. Simultaneous inhibition of BRAF and miR-3151 potentiates the effects on tumor cell growth. These data establish a link between mutated
BRAF
and the
TP53
pathway, allowing novel therapeutic approaches to be considered.
The
B-Raf proto-oncogene serine/threonine kinase
(
BRAF
) gene is the most frequently mutated gene in malignant melanoma (MM) and papillary thyroid cancer (PTC) and is causally involved in malignant cell transformation. Mutated BRAF is associated with an aggressive disease phenotype, thus making it a top candidate for targeted treatment strategies in MM and PTC. We show that
BRAF
mutations in both MM and PTC drive increased expression of oncomiR-3151, which is coactivated by the SP1/NF-κB complex. Knockdown of microRNA-3151 (miR-3151) with short hairpin RNAs reduces cell proliferation and increases apoptosis of MM and PTC cells. Using a targeted RNA sequencing approach, we mechanistically determined that miR-3151 directly targets TP53 and other members of the TP53 pathway. Reducing miR-3151’s abundance increases TP53’s mRNA and protein expression and favors its nuclear localization. Consequently, knockdown of miR-3151 also leads to caspase-3–dependent apoptosis. Simultaneous inhibition of aberrantly activated BRAF and knockdown of miR-3151 potentiates the effects of sole BRAF inhibition with the BRAF inhibitor vemurafenib and may provide a novel targeted therapeutic approach in
BRAF
-mutated MM and PTC patients. In conclusion, we identify miR-3151 as a previously unidentified player in MM and PTC pathogenesis, which is driven by BRAF-dependent and BRAF-independent mechanisms. Characterization of TP53 as a downstream effector of miR-3151 provides evidence for a causal link between
BRAF
mutations and TP53 inactivation.

Proceedings of the National Academy of Sciences - PNAS, 2015-12, Vol.112 (49), p.E6744-E6751

MicroRNA-3151 inactivates TP53 in BRAF-mutated human malignancies

Neuroblastoma rat sarcoma (RAS) viral oncogene homolog (NRAS), a small GTPase, is one of the most thoroughly studied oncogenes that controls cell growth, differentiation, and survival by facilitating signal transduction. Here, we identify four novel naturally occurring NRAS isoforms (isoforms 2—5) in addition to the canonical isoform (isoform 1). Expression analyses performed on a panel of several different human malignancies and matching normal tissue revealed distinct isoform expression patterns. Two of the novel isoforms were found in the nucleus and cytoplasm, whereas the others were exclusively cytoplasmic. The isoforms varied in their binding affinities to known downstream targets and differentially regulated the RAS signaling pathway. Strikingly, forced expression of isoform 5, which encodes only a 20-aa peptide, led to increased cell proliferation and to transformation by activation of known NRAS targets. These discoveries open new avenues in the study of NRAS.

Proceedings of the National Academy of Sciences - PNAS, 2014-03, Vol.111 (11), p.4179-4184

NRAS isoforms differentially affect downstream pathways, cell growth, and cell transformation

TheB-Raf proto-oncogene serine/threonine kinase(BRAF) gene is the most frequently mutated gene in malignant melanoma (MM) and papillary thyroid cancer (PTC) and is causally involved in malignant cell transformation. Mutated BRAF is associated with an aggressive disease phenotype, thus making it a top candidate for targeted treatment strategies in MM and PTC. We show thatBRAFmutations in both MM and PTC drive increased expression of oncomiR-3151, which is coactivated by the SP1/NF-κB complex. Knockdown of microRNA-3151 (miR-3151) with short hairpin RNAs reduces cell proliferation and increases apoptosis of MM and PTC cells. Using a targeted RNA sequencing approach, we mechanistically determined that miR-3151 directly targets TP53 and other members of the TP53 pathway. Reducing miR-3151’s abundance increases TP53’s mRNA and protein expression and favors its nuclear localization. Consequently, knockdown of miR-3151 also leads to caspase-3–dependent apoptosis. Simultaneous inhibition of aberrantly activated BRAF and knockdown of miR-3151 potentiates the effects of sole BRAF inhibition with the BRAF inhibitor vemurafenib and may provide a novel targeted therapeutic approach inBRAF-mutated MM and PTC patients. In conclusion, we identify miR-3151 as a previously unidentified player in MM and PTC pathogenesis, which is driven by BRAF-dependent and BRAF-independent mechanisms. Characterization of TP53 as a downstream effector of miR-3151 provides evidence for a causal link betweenBRAFmutations and TP53 inactivation.

MicroRNA-3151 inactivates TP53 inBRAF-mutated human malignancies

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Item 14: Walkabout magazine : New South Wales photographs [opal mining; early NSW buildings; fishing; bush and golf]

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MicroRNA-3151 inactivates TP53 in BRAF-mutated human malignancies

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NRAS isoforms differentially affect downstream pathways, cell growth, and cell transformation

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MicroRNA-3151 inactivates TP53 inBRAF-mutated human malignancies

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