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ArticlesPeer ReviewedFull Text
Glutamine-free mammalian expression of recombinant glycoproteins with uniform isotope labeling: an application for NMR analysis of pharmaceutically relevant Fc glycoforms of human immunoglobulin G1
Author / Creator:Yanaka, Saeko ; Yagi, Hirokazu ; Yogo, Rina ; Onitsuka, Masayoshi ; Kato, Koichi
Publisher:Dordrecht: Springer Netherlands
Journal title:Journal of biomolecular NMR, 2022-04, Vol.76 (1-2), p.17-22
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ArticlesPeer ReviewedFull Text
Secretion analysis of intracellular “difficult-to-express” immunoglobulin G (IgG) in Chinese hamster ovary (CHO) cells
Author / Creator:Kaneyoshi, Kohei ; Kuroda, Kouki ; Uchiyama, Keiji ; Onitsuka, Masayoshi ; Yamano-Adachi, Noriko ; Koga, Yuichi ; Omasa, Takeshi
Publisher:Dordrecht: Springer Netherlands
Journal title:Cytotechnology (Dordrecht), 2019-02, Vol.71 (1), p.305-316
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ArticlesPeer ReviewedFull Text
Enhanced IgG1 production by overexpression of nuclear factor kappa B inhibitor zeta (NFKBIZ) in Chinese hamster ovary cells
Author / Creator:Onitsuka, Masayoshi ; Kinoshita, Yukie ; Nishizawa, Akitoshi ; Tsutsui, Tomomi ; Omasa, Takeshi
Publisher:Dordrecht: Springer Netherlands
Journal title:Cytotechnology (Dordrecht), 2018-04, Vol.70 (2), p.675-685
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ArticlesPeer ReviewedFull Text
Rapid construction of transgene-amplified CHO cell lines by cell cycle checkpoint engineering
Author / Creator:Lee, Kyoung Ho ; Onitsuka, Masayoshi ; Honda, Kohsuke ; Ohtake, Hisao ; Omasa, Takeshi
Publisher:Berlin/Heidelberg: Springer-Verlag
Journal title:Applied microbiology and biotechnology, 2013-07, Vol.97 (13), p.5731-5741
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ArticlesPeer ReviewedFull Text
Build-up functionalization of anti-EGFR × anti-CD3 bispecific diabodies by integrating high-affinity mutants and functional molecular formats
Author / Creator:Asano, Ryutaro ; Hosokawa, Katsuhiro ; Taki, Shintaro ; Konno, Shota ; Shimomura, Ippei ; Ogata, Hiromi ; Okada, Mai ; Arai, Kyoko ; Onitsuka, Masayoshi ; Omasa, Takeshi ; Nakanishi, Takeshi ; Umetsu, Mitsuo ; Kumagai, Izumi
Publisher:London: Nature Publishing Group UK
Journal title:Scientific reports, 2020-03, Vol.10 (1), p.4913-4913, Article 4913
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ArticlesPeer ReviewedFull Text
Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of α2,6-sialyltransferase derived from Chinese hamster ovary cells
Author / Creator:Onitsuka, Masayoshi ; Kim, Wook-Dong ; Ozaki, Hiroyuki ; Kawaguchi, Akira ; Honda, Kohsuke ; Kajiura, Hiroyuki ; Fujiyama, Kazuhito ; Asano, Ryutaro ; Kumagai, Izumi ; Ohtake, Hisao ; Omasa, Takeshi
Publisher:Berlin/Heidelberg: Springer-Verlag
Journal title:Applied microbiology and biotechnology, 2012-04, Vol.94 (1), p.69-80
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ArticlesPeer ReviewedFull Text
Silkworm Pupae Function as Efficient Producers of Recombinant Glycoproteins with Stable-Isotope Labeling
Author / Creator:Yagi, Hirokazu ; Yanaka, Saeko ; Yogo, Rina ; Ikeda, Akari ; Onitsuka, Masayoshi ; Yamazaki, Toshio ; Kato, Tatsuya ; Park, Enoch Y. ; Yokoyama, Jun ; Kato, Koichi
Publisher:Switzerland: MDPI AG
Journal title:Biomolecules (Basel, Switzerland), 2020-10, Vol.10 (11), p.1482
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ArticlesPeer ReviewedFull Text
Increased antibody productivity in Chinese Hamster Ovary cells through induction of chromosomal instability by cell fusion
Author / Creator:Lai, YuanShan ; Yamano, Noriko ; Onitsuka, Masayoshi ; Omasa, Takeshi
Publisher:London: BioMed Central
Journal title:BMC proceedings, 2015-12, Vol.9 (S9), p.P11-P11, Article P11
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ArticlesPeer ReviewedFull Text
Stability difference of each chromosome in Chinese Hamster Ovary cell line
Author / Creator:Yamano, Noriko ; Kumamoto, Toshitaka ; Takahashi, Mai ; Frank, Jana ; Onitsuka, Masayoshi ; Omasa, Takeshi
Publisher:London: BioMed Central
Journal title:BMC proceedings, 2015-12, Vol.9 (S9), p.P1-P1, Article P1
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ArticlesPeer ReviewedFull Text
Construction of a gene knockout CHO cell line using a simple gene targeting method
Author / Creator:Aga, Marina ; Yamano, Noriko ; Kumamoto, Toshitaka ; Frank, Jana ; Onitsuka, Masayoshi ; Omasa, Takeshi
Publisher:London: BioMed Central
Journal title:BMC proceedings, 2015-12, Vol.9 (S9), p.P2-P2, Article P2
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ArticlesPeer ReviewedFull Text
Chemical chaperone suppresses the antibody aggregation in CHO cell culture
Author / Creator:Onitsuka, Masayoshi ; Tatsuzawa, Miki ; Noda, Masahiro ; Omasa, Takeshi
Publisher:London: BioMed Central
Journal title:BMC proceedings, 2013-12, Vol.7 (S6), p.P68-P68, Article P68
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ArticlesPeer ReviewedFull Text
The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III
Author / Creator:Yogo, Rina ; Yamaguchi, Yuki ; Watanabe, Hiroki ; Yagi, Hirokazu ; Satoh, Tadashi ; Nakanishi, Mahito ; Onitsuka, Masayoshi ; Omasa, Takeshi ; Shimada, Mari ; Maruno, Takahiro ; Torisu, Tetsuo ; Watanabe, Shio ; Higo, Daisuke ; Uchihashi, Takayuki ; Yanaka, Saeko ; Uchiyama, Susumu ; Kato, Koichi
Publisher:London: Nature Publishing Group UK
Journal title:Scientific reports, 2019-08, Vol.9 (1), p.11957-10, Article 11957
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ArticlesPeer ReviewedFull Text
Dynamical analysis of antibody aggregation in the CHO cell culture with Thermo Responsive Protein A (TRPA) column
Author / Creator:Noda, Masahiro ; Onitsuka, Masayoshi ; Tatsuzawa, Miki ; Koguma, Ichiro ; Omasa, Takeshi
Publisher:London: BioMed Central
Journal title:BMC proceedings, 2013-12, Vol.7 (S6), p.P69-P69, Article P69
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ArticlesPeer ReviewedFull Text
Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of alpha 2,6-sialyltransferase derived from Chinese hamster ovary cells
Author / Creator:Onitsuka, Masayoshi ; Kim, Wook-Dong ; Ozaki, Hiroyuki ; Kawaguchi, Akira ; Honda, Kohsuke ; Kajiura, Hiroyuki ; Fujiyama, Kazuhito ; Asano, Ryutaro ; Kumagai, Izumi ; Ohtake, Hisao ; Omasa, Takeshi
Journal title:Applied microbiology and biotechnology, 2012-04, Vol.94 (1), p.69-80
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Glutamine-free mammalian expression of recombinant glycoproteins with uniform isotope labeling: an application for NMR analysis of pharmaceutically relevant Fc glycoforms of human immunoglobulin G1

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Secretion analysis of intracellular “difficult-to-express” immunoglobulin G (IgG) in Chinese hamster ovary (CHO) cells

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Enhanced IgG1 production by overexpression of nuclear factor kappa B inhibitor zeta (NFKBIZ) in Chinese hamster ovary cells

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Rapid construction of transgene-amplified CHO cell lines by cell cycle checkpoint engineering

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Build-up functionalization of anti-EGFR × anti-CD3 bispecific diabodies by integrating high-affinity mutants and functional molecular formats

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Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of α2,6-sialyltransferase derived from Chinese hamster ovary cells

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Silkworm Pupae Function as Efficient Producers of Recombinant Glycoproteins with Stable-Isotope Labeling

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Increased antibody productivity in Chinese Hamster Ovary cells through induction of chromosomal instability by cell fusion

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Stability difference of each chromosome in Chinese Hamster Ovary cell line

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Construction of a gene knockout CHO cell line using a simple gene targeting method

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Chemical chaperone suppresses the antibody aggregation in CHO cell culture

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The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III

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Dynamical analysis of antibody aggregation in the CHO cell culture with Thermo Responsive Protein A (TRPA) column

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Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of alpha 2,6-sialyltransferase derived from Chinese hamster ovary cells

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