Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors
Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors
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United States: Public Library of Science
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English
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United States: Public Library of Science
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High-throughput genetic screens are powerful methods to identify genes linked to a given phenotype. The catalytic null mutant of the Cas9 RNA-guided nuclease (dCas9) can be conveniently used to silence genes of interest in a method also known as CRISPRi. Here, we report a genome-wide CRISPR-dCas9 screen using a starting pool of ~ 92,000 sgRNAs whic...
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Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors
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TN_cdi_plos_journals_2251023846
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_plos_journals_2251023846
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ISSN
1553-7404,1553-7390
E-ISSN
1553-7404
DOI
10.1371/journal.pgen.1007749
