A tiling-deletion-based genetic screen for cis-regulatory element identification in mammalian cells
A tiling-deletion-based genetic screen for cis-regulatory element identification in mammalian cells
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Author / Creator
Diao, Yarui , Fang, Rongxin , Li, Bin , Meng, Zhipeng , Yu, Juntao , Qiu, Yunjiang , Lin, Kimberly C , Huang, Hui , Liu, Tristin , Marina, Ryan J , Jung, Inkyung , Shen, Yin , Guan, Kun-Liang and Ren, Bing
Publisher
New York: Nature Publishing Group US
Journal title
Language
English
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Publisher
New York: Nature Publishing Group US
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Scope and Contents
Contents
CREST-seq allows functional screening of
cis
-regulatory elements through the use of sgRNA pairs to introduce tiled deletions in regions of interest. The analysis of a 2-megabase target region shows that promoters can act as distal enhancers for unrelated genes.
Millions of
cis
-regulatory elements are predicted to be present in the...
Alternative Titles
Full title
A tiling-deletion-based genetic screen for cis-regulatory element identification in mammalian cells
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TN_cdi_proquest_miscellaneous_1889381441
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_1889381441
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ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth.4264
