Proximity labeling in mammalian cells with TurboID and split-TurboID
Proximity labeling in mammalian cells with TurboID and split-TurboID
About this item
Full title
Author / Creator
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
Formats
Publication information
Publisher
London: Nature Publishing Group UK
Subjects
More information
Scope and Contents
Contents
This protocol describes the use of TurboID and split-TurboID in proximity labeling applications for mapping protein–protein interactions and subcellular proteomes in live mammalian cells. TurboID is an engineered biotin ligase that uses ATP to convert biotin into biotin–AMP, a reactive intermediate that covalently labels proximal proteins. Optimize...
Alternative Titles
Full title
Proximity labeling in mammalian cells with TurboID and split-TurboID
Authors, Artists and Contributors
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_proquest_miscellaneous_2457304909
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_2457304909
Other Identifiers
ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/s41596-020-0399-0
