Analysis of nuclear RNA interference in human cells by subcellular fractionation and Argonaute loadi...
Analysis of nuclear RNA interference in human cells by subcellular fractionation and Argonaute loading
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Publisher
London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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Contents
This protocol describes an optimized cell fractionation protocol for obtaining nuclei (or soluble and insoluble nuclear fractions) uncontaminated by cytoplasm or organelles— critical for studying nuclear RNAi or other nucleus-specific processes.
RNAi is well known for its ability to regulate gene expression in the cytoplasm of mammalian cells. I...
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Analysis of nuclear RNA interference in human cells by subcellular fractionation and Argonaute loading
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4251768
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4251768
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ISSN
1754-2189,1750-2799
E-ISSN
1750-2799
DOI
10.1038/nprot.2014.135
