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microRNA-1 regulates sarcomere formation and suppresses smooth muscle gene expression in the mammali...

microRNA-1 regulates sarcomere formation and suppresses smooth muscle gene expression in the mammali...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_7e574b2d00aa47e2b8ec607b379d5148

microRNA-1 regulates sarcomere formation and suppresses smooth muscle gene expression in the mammalian heart

About this item

Full title

microRNA-1 regulates sarcomere formation and suppresses smooth muscle gene expression in the mammalian heart

Publisher

England: eLife Sciences Publications Ltd

Journal title

eLife, 2013-11, Vol.2, p.e01323-e01323

Language

English

Formats

Publication information

Publisher

England: eLife Sciences Publications Ltd

More information

Scope and Contents

Contents

microRNA-1 (miR-1) is an evolutionarily conserved, striated muscle-enriched miRNA. Most mammalian genomes contain two copies of miR-1, and in mice, deletion of a single locus, miR-1-2, causes incompletely penetrant lethality and subtle cardiac defects. Here, we report that deletion of miR-1-1 resulted in a phenotype similar to that of the miR-1-2 mutant. Compound miR-1 knockout mice died uniformly before weaning due to severe cardiac dysfunction. miR-1-null cardiomyocytes had abnormal sarcomere organization and decreased phosphorylation of the regulatory myosin light chain-2 (MLC2), a critical cytoskeletal regulator. The smooth muscle-restricted inhibitor of MLC2 phosphorylation, Telokin, was ectopically expressed in the myocardium, along with other smooth muscle genes. miR-1 repressed Telokin expression through direct targeting and by repressing its transcriptional regulator, Myocardin. Our results reveal that miR-1 is required for postnatal cardiac function and reinforces the striated muscle phenotype by regulating both transcriptional and effector nodes of the smooth muscle gene expression network. DOI: http://dx.doi.org/10.7554/eLife.01323.001....

Alternative Titles

Full title

microRNA-1 regulates sarcomere formation and suppresses smooth muscle gene expression in the mammalian heart

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_7e574b2d00aa47e2b8ec607b379d5148

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_7e574b2d00aa47e2b8ec607b379d5148

Other Identifiers

ISSN

2050-084X

E-ISSN

2050-084X

DOI

10.7554/eLife.01323

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