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The role of protein dynamics in the evolution of new enzyme function
Author / Creator:Campbell, Eleanor ; Kaltenbach, Miriam ; Correy, Galen J ; Carr, Paul D ; Porebski, Benjamin T ; Livingstone, Emma K ; Afriat-Jurnou, Livnat ; Buckle, Ashley M ; Weik, Martin ; Hollfelder, Florian ; Tokuriki, Nobuhiko ; Jackson, Colin J
Publisher:New York: Nature Publishing Group US
Journal title:Nature chemical biology, 2016-11, Vol.12 (11), p.944-950
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Highly active enzymes by automated combinatorial backbone assembly and sequence design
Author / Creator:Lapidoth, Gideon ; Khersonsky, Olga ; Lipsh, Rosalie ; Dym, Orly ; Albeck, Shira ; Rogotner, Shelly ; Fleishman, Sarel J.
Publisher:London: Nature Publishing Group UK
Journal title:Nature communications, 2018-07, Vol.9 (1), p.2780-9, Article 2780
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Rational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase
Author / Creator:Jacquet, Pauline ; Hiblot, Julien ; Daudé, David ; Bergonzi, Céline ; Gotthard, Guillaume ; Armstrong, Nicholas ; Chabrière, Eric ; Elias, Mikael
Publisher:London: Nature Publishing Group UK
Journal title:Scientific reports, 2017-12, Vol.7 (1), p.16745-15, Article 16745
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Post-VX exposure treatment of rats with engineered phosphotriesterases
Author / Creator:Stigler, Lisa ; Köhler, Anja ; Koller, Marianne ; Job, Laura ; Escher, Benjamin ; Potschka, Heidrun ; Thiermann, Horst ; Skerra, Arne ; Worek, Franz ; Wille, Timo
Publisher:Berlin/Heidelberg: Springer Berlin Heidelberg
Journal title:Archives of toxicology, 2022-02, Vol.96 (2), p.571-583
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Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
Author / Creator:Emond, Stephane ; Petek, Maya ; Kay, Emily J. ; Heames, Brennen ; Devenish, Sean R. A. ; Tokuriki, Nobuhiko ; Hollfelder, Florian
Publisher:London: Nature Publishing Group UK
Journal title:Nature communications, 2020-07, Vol.11 (1), p.3469-14, Article 3469
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A family of metal-dependent phosphatases implicated in metabolite damage-control
Author / Creator:Huang, Lili ; Khusnutdinova, Anna ; Nocek, Boguslaw ; Brown, Greg ; Xu, Xiaohui ; Cui, Hong ; Petit, Pierre ; Flick, Robert ; Zallot, Rémi ; Balmant, Kelly ; Ziemak, Michael J ; Shanklin, John ; de Crécy-Lagard, Valérie ; Fiehn, Oliver ; Gregory, Jesse F ; Joachimiak, Andrzej ; Savchenko, Alexei ; Yakunin, Alexander F ; Hanson, Andrew D
Publisher:New York: Nature Publishing Group US
Journal title:Nature chemical biology, 2016-08, Vol.12 (8), p.621-627
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Catalytic activity and stereoselectivity of engineered phosphotriesterases towards structurally different nerve agents in vitro
Author / Creator:Köhler, Anja ; Escher, Benjamin ; Job, Laura ; Koller, Marianne ; Thiermann, Horst ; Skerra, Arne ; Worek, Franz
Publisher:Berlin/Heidelberg: Springer Berlin Heidelberg
Journal title:Archives of toxicology, 2021-08, Vol.95 (8), p.2815-2823
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Conformational sampling, catalysis, and evolution of the bacterial phosphotriesterase
Author / Creator:Jackson, C.J ; Foo, J.-L ; Tokuriki, N ; Afriat, L ; Carr, P.D ; Kim, H.-K ; Schenk, G ; Tawfik, D.S ; Ollis, D.L
Publisher:United States: National Academy of Sciences
Journal title:Proceedings of the National Academy of Sciences - PNAS, 2009-12, Vol.106 (51), p.21631-21636
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Differential Active Site Loop Conformations Mediate Promiscuous Activities in the Lactonase SsoPox
Author / Creator:Hiblot, Julien ; Gotthard, Guillaume ; Elias, Mikael ; Chabriere, Eric
Publisher:United States: Public Library of Science
Journal title:PloS one, 2013-09, Vol.8 (9), p.e75272-e75272
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Protecting enzymatic function through directed packaging into bacterial outer membrane vesicles
Author / Creator:Alves, Nathan J. ; Turner, Kendrick B. ; Medintz, Igor L. ; Walper, Scott A.
Publisher:London: Nature Publishing Group UK
Journal title:Scientific reports, 2016-04, Vol.6 (1), p.24866-24866, Article 24866
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An atypical phosphodiesterase capable of degrading haloalkyl phosphate diesters from Sphingobium sp. strain TCM1
Author / Creator:Abe, Katsumasa ; Mukai, Naoko ; Morooka, Yuka ; Makino, Takeshi ; Oshima, Kenji ; Takahashi, Shouji ; Kera, Yoshio
Publisher:London: Nature Publishing Group UK
Journal title:Scientific reports, 2017-06, Vol.7 (1), p.2842-9, Article 2842
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High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and...
Author / Creator:Restaino, Odile Francesca ; Borzacchiello, Maria Giovanna ; Scognamiglio, Ilaria ; Fedele, Luigi ; Alfano, Alberto ; Porzio, Elena ; Manco, Giuseppe ; De Rosa, Mario ; Schiraldi, Chiara
Publisher:England: BioMed Central Ltd
Journal title:BMC biotechnology, 2018-03, Vol.18 (1), p.18-18, Article 18
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The 'evolvability' of promiscuous protein functions
Author / Creator:Aharoni, Amir ; Gaidukov, Leonid ; Khersonsky, Olga ; Gould, Stephen McQ ; Roodveldt, Cintia ; Tawfik, Dan S
Publisher:New York: Nature Publishing Group US
Journal title:Nature genetics, 2005-01, Vol.37 (1), p.73-76
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Reverse evolution leads to genotypic incompatibility despite functional and active site convergence
Author / Creator:Kaltenbach, Miriam ; Jackson, Colin J ; Campbell, Eleanor C ; Hollfelder, Florian ; Tokuriki, Nobuhiko
Publisher:England: eLife Sciences Publications, Ltd
Journal title:eLife, 2015-08, Vol.4
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Computational Design of Phosphotriesterase Improves V‐Agent Degradation Efficiency
Author / Creator:Kronenberg, Jacob ; Chu, Stanley ; Olsen, Andrew ; Britton, Dustin ; Halvorsen, Leif ; Guo, Shengbo ; Lakshmi, Ashwitha ; Chen, Jason ; Kulapurathazhe, Maria Jinu ; Baker, Cetara A. ; Wadsworth, Benjamin C. ; Van Acker, Cynthia J. ; Lehman, John G. ; Otto, Tamara C. ; Renfrew, P. Douglas ; Bonneau, Richard ; Montclare, Jin Kim
Publisher:Germany: John Wiley & Sons, Inc
Journal title:ChemistryOpen (Weinheim), 2024-07, Vol.13 (7), p.e202300263-n/a
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A thermostable phosphotriesterase from the archaeon Sulfolobus solfataricus: cloning, overexpression and properties
Author / Creator:Merone, Luigia ; Mandrich, Luigi ; Rossi, Mosè ; Manco, Giuseppe
Publisher:Germany: Springer Nature B.V
Journal title:Extremophiles : life under extreme conditions, 2005-08, Vol.9 (4), p.297-305
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Diminishing returns and tradeoffs constrain the laboratory optimization of an enzyme
Author / Creator:Tokuriki, Nobuhiko ; Jackson, Colin J. ; Afriat-Jurnou, Livnat ; Wyganowski, Kirsten T. ; Tang, Renmei ; Tawfik, Dan S.
Publisher:London: Nature Publishing Group UK
Journal title:Nature communications, 2012-12, Vol.3 (1), p.1257, Article 1257
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Catalytic efficiencies of directly evolved phosphotriesterase variants with structurally different organophosphorus compounds in vitro
Author / Creator:Goldsmith, Moshe ; Eckstein, Simone ; Ashani, Yacov ; Greisen, Per ; Leader, Haim ; Sussman, Joel L. ; Aggarwal, Nidhi ; Ovchinnikov, Sergey ; Tawfik, Dan S. ; Baker, David ; Thiermann, Horst ; Worek, Franz
Publisher:Berlin/Heidelberg: Springer Berlin Heidelberg
Journal title:Archives of toxicology, 2016-11, Vol.90 (11), p.2711-2724
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Engineering a Mesoporous Silicon Nanoparticle Cage to Enhance Performance of a Phosphotriesterase Enzyme for Degradation of VX Nerve Agent
Author / Creator:Lu, Yi‐Sheng ; Moreno, Eduardo Reynoso ; Huang, Yubin ; Fan, Ruhan ; Tucker, Ashley T. ; Wright, Linnzi K. ; Evans, Ronald A. ; Ahern, Brooke M. ; Owens, Donald E. ; Chappell, Stephen A. ; Christensen, Dale J. ; Dresios, John ; Sailor, Michael J.
Publisher:Germany: John Wiley & Sons, Inc
Journal title:Advanced science, 2024-12, Vol.11 (48), p.e2409535-n/a
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Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity
Author / Creator:Kaltenbach, Miriam ; Emond, Stephane ; Hollfelder, Florian ; Tokuriki, Nobuhiko
Publisher:United States: Public Library of Science
Journal title:PLoS genetics, 2016-10, Vol.12 (10), p.e1006305
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The role of protein dynamics in the evolution of new enzyme function

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Highly active enzymes by automated combinatorial backbone assembly and sequence design

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Rational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase

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Post-VX exposure treatment of rats with engineered phosphotriesterases

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Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

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A family of metal-dependent phosphatases implicated in metabolite damage-control

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Catalytic activity and stereoselectivity of engineered phosphotriesterases towards structurally different nerve agents in vitro

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Conformational sampling, catalysis, and evolution of the bacterial phosphotriesterase

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Differential Active Site Loop Conformations Mediate Promiscuous Activities in the Lactonase SsoPox

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Protecting enzymatic function through directed packaging into bacterial outer membrane vesicles

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An atypical phosphodiesterase capable of degrading haloalkyl phosphate diesters from Sphingobium sp. strain TCM1

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High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and...

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The 'evolvability' of promiscuous protein functions

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Reverse evolution leads to genotypic incompatibility despite functional and active site convergence

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Computational Design of Phosphotriesterase Improves V‐Agent Degradation Efficiency

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A thermostable phosphotriesterase from the archaeon Sulfolobus solfataricus: cloning, overexpression and properties

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Diminishing returns and tradeoffs constrain the laboratory optimization of an enzyme

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Catalytic efficiencies of directly evolved phosphotriesterase variants with structurally different organophosphorus compounds in vitro

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Engineering a Mesoporous Silicon Nanoparticle Cage to Enhance Performance of a Phosphotriesterase Enzyme for Degradation of VX Nerve Agent

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Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity

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